Metabolite Profiling of the Environmental-Controlled Growth of Marsilea crenata Presl. and Its In Vitro and In Silico Antineuroinflammatory Properties

This study was aimed to evaluate the metabolite contents and antineuroinflammatory potential of Marsilea crenata Presl. grown under a controlled environmental condition. The test has been carried out in vitro using ethanolic extract M. leaves on HMC3 microglia cells. An silico approach applied predict active compounds extract. cells were induced with IFNγ create prolonged inflammatory conditions then treated 96% 62.5, 125, 250 μg/mL. expression MHC II analyzed ICC method CLSM instrument. Metabolites profiled UPLC-QToF-MS/MS instrument MassLynx 4.1 software. In evaluation conducted molecular docking 3OLS protein PyRx 0.8 software, physicochemical properties SwissADME webtool. could reduce all concentrations values 97.458, 139.574, 82.128 AU. result profiling found 79 showed that 19 gave agonist interaction toward 3OLS, three met parameters analysis. environmental-controlled growth activity predicted contain some phytoestrogen which act as agonists.